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6 museums selected in this guide.

The Uffizi Gallery is one of the world's most important art museums, housing the Medici family's unrivalled collection of Renaissance masterworks. Botticelli's 'Birth of Venus' and 'Primavera' anchor a collection that spans Giotto to Caravaggio across 101 rooms.

Palazzo Pitti is a colossal Renaissance palace on the Oltrarno, serving as the Medici's primary residence from 1549. It houses five museums—including the Palatine Gallery with its Raphael and Titian masterpieces—and connects to the Boboli Gardens behind.

The Bargello is Florence's premier sculpture museum, housed in a 1255 fortress that served as the city's first town hall, then a prison. Its courtyard and halls display works by Donatello, Michelangelo, Cellini, and Giambologna.

The Galleria dell'Accademia is home to Michelangelo's David—the 5.17-metre marble colossus carved from a single block in 1501–1504. Beyond David, the gallery holds Michelangelo's unfinished 'Prisoners' and a rich collection of Florentine Gothic and Renaissance painting.
The Museo di San Marco occupies a 15th-century Dominican convent frescoed throughout by Fra Angelico. Each monk's cell contains an individual fresco designed as a meditation aid—among the most intimate and moving works of the early Renaissance.

The Museo Galileo houses one of the world's finest collections of scientific instruments, including Galileo's original telescopes and the lens with which he discovered Jupiter's moons. Over 1,000 instruments span the Renaissance to the 19th century.